Table 3 Results of preliminary Phytochemical Screening of Baissea axillaries leaves. Chromatographic results.
Secondary Metabolites | Baissea axillaries extracts | ||||
|---|---|---|---|---|---|
| Â | Â | Aqueous | Ethanolic | ||
Alkaloids | Â | Â | Â | Â | |
Dragendorff's reagent | + | + | |||
Wagner's reagent | + | + | |||
Mayer's reagent | + | + | |||
1% picric acid reagent | + | + | |||
Anthracene derivatives | Â | Â | Â | Â | |
Combined anthraquinones | - | - | |||
Free anthraquinones | - | - | |||
Tannins | Â | Â | Â | Â | |
Phenazone test | + | + | |||
Iron complex test | + | + | |||
Formaldehyde test | + | + | |||
Modified iron complex test | + | + | |||
Saponin glycosides | Â | Â | Â | Â | |
Frothing test | - | - | |||
Haemolysis test | - | - | |||
Cardiac glycosides | Â | Â | Â | Â | |
Keller killiani test | - | - | |||
Kedde test | - | - | |||
Cyanogenetic glycosides | Â | Â | Â | Â | |
Sodium picrate test paper | + | + | |||
Thin layer chromatography | |||||
Extract | No of spots | Colour in daylight | Colour in UV | Colour after spraying with Dragendorff | R f value |
Aqueous | 2 | colourless | Light green fluorescent | reddish brown | 0.55 0.72 |
Ethanolic | 2 | colourless | Light green fluorescent | reddish brown | 0.53 0.71 |
Paper chromatography | |||||
Extract | No of spots | Colour in daylight | Colour in UV | Colour after spraying with Ferric chloride | R f value |
Aqueous | 1 | colourless | green fluorescent | Violet colour | 0.73 |
Ethanolic | 1 | colourless | green fluorescent | Violet colour | 0.72 |