Effects of total chikusetsusaponins, various chikusetsusaponins and orlistat (a lipase inhibitor) on pancreatic lipase activity. Values are means ± S.E. of 4 experiments. a) Solid circles, methanol extract; open circles, total chikusetsusaponins; open squares, orlistat (a lipase inhibitor) was used as a positive control. b) Open circles, chikusetsusaponin III; solid circles, chikusetsusaponin IV; open squares, 28-deglucosyl-chikusetsusaponin IV; solid squares, 28-deglucosyl-chikusetsusaponin V.