Western blot analysis for nitrotyrosine formation in brain, liver and TA muscle. M, molecular mass markers. Lane 1, rested control rats. Lane 2, rested control rats treated with HCE. Lane 3, acutely exercised rats. Lane 4, acutely exercised rats treated with HCE 24, 12 and 0.5 h before acute exercise. Nitrotyrosine formation was analyzed at 1 h (TA muscle), 6 h (liver) and 48 h (brain) after exercise. The results are representative of three independent experiments.