Effect of the HCE from P. emarginatus on nitrite levels in brain, liver and TA muscle after acute exercise. (A) Nitrite levels were estimated by the Griess reaction in tissue homogenates of rested control rats and at 1 h (Exer 1 h), 6 h (Exer 6 h) and 48 h (Exer 48 h) after exercise. (B) Nitrite was determined in tissue homogenates of rested control rats (1); acutely exercised rats (2); rested rats treated with HCE (3); acutely exercised rats treated with HCE 0.5 h before exercise (4) acutely exercised rats treated with HCE 0.5 h after exercise (5). Nitrite levels were estimated at 1 h (TA muscle), 6 h (liver) and 48 h (brain) after exercise. Each column shows the mean ± SE of 8–10 rats. Significance was determined with one-way ANOVA followed by the Tukey-Kramer test. Columns with different letters differed significantly (p < 0.05).