Table 2 Showing frequency distribution of mitotic indices (Ml) in 5000 cells, chromosome aberration (CA) in 500 cells, micronuclei (MN) in polychromatic (PCE) and normochromatic (NCE) erythrocytes in 5000 cells and sperm head anomaly (SHA) in 5000 sperm in mice treated with p-Dimethylaminoazobenzene (DAB) + Phenobarbital (PB), DAB + PB + Chelidonium (Ch)-30 and DAB + PB + Chelidonium (Ch)-200 and their Phenobarbital treated and normal controls. Equal number of cells/sperms observed from each of 5 mice of a set.
From: Effect of a homeopathic drug, Chelidonium, in amelioration of p-DAB induced hepatocarcinogenesis in mice
| | |
Mitotic Index
|
Chromosome Aberration
|
Micronucleated Erythrocytes
|
Sperm Head Anomaly
|
|---|
|
Fixation intervals (days)
|
Series
|
% of Ml (% ± SE)
|
% of Prot.
|
% of Major CA
|
% of Other CA
|
Total CA (% ± SE)
|
% of Prot.
|
% of MN in PCE
|
% of MN in NCE
|
P/N
|
Total MN (% ± SE)
|
% of Prot.
|
Total SHA (% ± SE)
|
% of Prot.
|
|---|
| |
Normal
|
1.32 ± 0.08
| |
1.4
|
1.4
|
2.80 ± 0.58
| |
0.04
|
0.26
|
0.89
|
0.16 ± 0.05
| |
0.44 ± 0.12
| |
| |
PB
|
2.10 ± 0.48
| |
1.6
|
1.8
|
3.40 ± 0.98
| |
0.14
|
0.28
|
0.74
|
0.16 ± 0.05
| |
1.08 ± 0.24
| |
|
60
|
DAB + PB
|
8.70 ± 0.79
| |
5.4
|
10.4
|
15.8 ± 2.11
| |
0.53
|
0.88
|
0.84
|
0.72 ± 0.14
| |
2.62 ± 0.28
| |
| |
DAB + PB + Ch-30
|
4.66 ± 0.34
|
4.04b
|
4.4
|
4.6
|
9.0 ± 0.78
|
6.8a
|
0.41
|
0.61
|
0.79
|
0.52 ± 0.09
|
0.20
|
1.66 ± 0.30
|
0.96a
|
| |
DAB + PB + Ch-200
|
3.36 ± 0.37
|
5.34c
|
4.6
|
8.2
|
12.8 ± 3.87
|
3.0
|
0.45
|
0.26
|
0.497
|
0.32 ± 0.08
|
0.42a
|
0.8 ± 0.11
|
1.82c
|
| |
Normal
|
1.32 ± 0.08
| |
1.4
|
1.4
|
2.80 ± 0.58
| |
0.04
|
0.26
|
0.89
|
0.16 ± 0.05
| |
0.44 ± 0.12
| |
| |
PB
|
1.58 ± 0.14
| |
2.2
|
3.0
|
5.20 ± 0.86
| |
0.13
|
0.22
|
0.83
|
0.180.08
| |
0.52 ± 0.09
| |
|
90
|
DAB + PB
|
6.40 ± 1.14
| |
6.6
|
4.2
|
10.8 ± 1.20
| |
1.17
|
0.27
|
0.80
|
0.67 ± 0.10
| |
2.90 ± 0.27
| |
| |
DAB + PB + Ch-30
|
3.08 ± 0.29
|
3.3a
|
4.4
|
4.8
|
9.20 ± 1.86
|
1.6
|
0.24
|
0.55
|
1.33
|
0.37 ± 0.10
|
0.16
|
2.20 ± 0.26
|
0.70
|
| |
DAB + PB + Ch-200
|
2.90 ± 0.34
|
3.5a
|
4.6
|
5.6
|
10.2 ± 1.56
|
0.6
|
0.29
|
0.48
|
1.16
|
0.38 ± 0.11
|
0.16
|
1.66 ± 0.16
|
1.24b
|
| |
Normal
|
1.32 ± 0.08
| |
1.4
|
1.4
|
2.80 ± 0.58
| |
0.04
|
0.26
|
0.89
|
0.16 ± 0.05
| |
0.44 ± 0.12
| |
| |
PB
|
2.12 ± 0.30
| |
2.4
|
4.2
|
6.60 ± 0.93
| |
0.21
|
0.11
|
0.91
|
0.16 ± 0.08
|
1.12 ± 0.12
|
|
120
|
DAB + PB
|
8.20 ± 0.26
| |
8.6
|
13.0
|
22.4 ± 0.25
| |
0.63
|
0.54
|
0.80
|
0.58 ± 0.15
| |
1.58 ± 0.31
| |
| |
DAB + PB + Ch-30
|
8.72 ± 0.65
|
-0.52
|
7.8
|
7
|
14.8 ± 2.18
|
7.6b
|
0.49
|
0.27
|
0.34
|
0.32 ± 0.08
|
0.32
|
0.40 ± 0.12
|
1.18b
|
| |
DAB + PB + Ch-200
|
6.44 ± 0.35
|
1.8b
|
3.8
|
4.2
|
8.0 ± 0.32
|
14.4c
|
0.12
|
0.19
|
2.18
|
0.14 ± 0.06
|
0.43a
|
0.38 ± 0.07
|
1.20b
|
-
Major Types of CA include: Break; Centric fusion; Translocation; Fragment; Pulverisation; Ring; Terminal association; Polyploidy.;Aneuploidy. Other types of CA include: Erosion, Constriction, Precocious centromeric separation, Centromericstrecthing, Stickiness, C-mitotic effect. SE= standard error, a= p < 0.05, b= p < 0.01, c= p < 0.001. Prot.= Protection given by the drug
