Figure 7

ASIC3 agonist (acidified saline) injection at ST36 yielded no analgesic effect. The behavioral test design was similar to that of MA; one difference was that acidified saline was injected at different pH values in a volume of 10 μL into ST36 only. (A) In within-group tests, injections of acidified saline at different pH values did not yield significant differences on D3 post and on D4. (B and C) No significant difference was observed between pH 7.4 normal saline (N/S) injection and acidified saline injection on D3 post and on D4. Within-group tests and between-group tests were designed similar to the MA behavioral test (Mann–Whitney rank sum test, n = 9–15). Data are means ± S.E.M.