Figure 4

Immunofluorescence of TRPV1, TRPV4, and ASIC3 at ST36. TRPV1 (A), TRPV4 (B), and ASIC3 (C) were primarily expressed in muscle fibers (M), nerve (arrow), and subcutaneous cells (arrowhead) (as assessed using immunofluorescence). Immunofluorescence results correlated with the patterns observed in western blots. TRPV1 and ASIC3 showed evident expression in muscle and epimysium (between green lines), and TRPV4 was evident in subcutaneous cells. Note that stronger fluorescence was observed at the cell margin in muscles, which is in accordance with their role as membrane channels. (A) In particular, TRPV1 was expressed more strongly at a very thin layer located beneath the epimysium. Scale bars are as shown. (D), negative controls (no primary antibody).