Effects of EA at acupoints on pp90RSK, active caspase-3-NeuN, and TUNEL expression. (A) Representative photographs showed pp90RSK expression, (B) active caspase-3 (red) colocalizing with NeuN (blue), and (C) TUNEL-positive cells in the ischemic cortex of the sham, model, EA, non-acup, and U0126 + EA groups after 3 d of reperfusion. N, negative control stain. Arrows in (A) and (C) indicate pp90RSK- and TUNEL-positive cells, respectively. Arrow in (B) indicates active caspase-3-NeuN double-labeled cells (purple), shown at higher magnification in the bottom right panel. Scale bar = 50 μm.