Effect of EA at acupoints on BDNF expression in the ischemic cortex. (A) Representative photograph showed a brain coronal section (TTC stain) from posterior bregma 0.92 mm. The dotted line square indicates the area of evaluation of immunopositive cells. C, the ischemic area of the cortex. Dotted line square = 1 mm2. (B) Representative photographs showed BDNF expression in the ischemic cortex of the sham, model, EA, non-acup, and U0126 + EA groups after 3 d of reperfusion. N, negative control stain. Arrow indicates a BDNF-positive cell. Scale bar = 50 μm.