Figure 3
Effect of CWE on scavenger receptor activity. A-D: Mouse peritoneal macrophages were cultured with or without fucoidan (75 μg/ml) for 24 h and then incubated with Alexa488-acetyl LDL for 2 h. Cellular uptake of Alexa488-acetyl LDL was analyzed by flow cytometry. A, cells without Alexa488-acetyl LDL; B, cells with Alexa488-acetyl LDL; C, cells with Alexa488-acetyl LDL in the presence of fucoidan. E-G: THP-1 cells were stimulated with PMA (100 nM) for 24 h in the presence of CWE and then incubated with Alexa488-acetyl LDL for 16 h. E, cells treated with PMA only. F, cells treated with PMA plus CWE (100 μg/ml). H-J: Mouse peritoneal macrophages were cultured with CWE for 24 h and then incubated with Alexa488-acetyl LDL for 2 h. D, G, J: Results are presented as mean fluorescence intensity (MFI) ± SD of three independent assays. * p <0.05, ** p < 0.01 indicating significant difference from cells without treatment.