Figure 4

Effects of S-A144 on cell cycle progression and cell cycle-related protein expression in PDGF-BB-stimulated VSMCs. VSMCs cultured in serum-starved medium were stimulated with 25 ng/mL PDGF-BB, followed by the addition of various concentrations of S-A144 (100–500 μg/mL) or S-AOR (500 μg/mL) to determine the effects on DNA synthesis. (A) Cell cycle progression data are representative of three independent experiments. (B) CDKs, cyclins and PCNA expression were measured by SDS-PAGE and immunoblotting; total β-actin was used for normalisation. Immunoblots were analysed by densitometry; values indicate the level of expression relative to controls. Data are representative of three independent experiments. All values are expressed as the mean ± S.E.M.