Figure 2

Effect of S-A144 on VSMC proliferation and viability. VSMCs cultured in serum-free medium were stimulated with 25 ng/mL PDGF-BB for 24 h, and the effect of various concentrations of S-A144 (100–500 μg/mL) or S-AOR (500 μg/mL) on cell proliferation and viability was measured. (A) Cell proliferation was determined using a colourimetric WST-1 assay read at 450 nm (n = 4). (B) Cells were counted using a haemocytometer (n = 3). (C) VSMCs were treated with 500 ng/mL S-A144 for 24 h; cell viability was determined using a WST-1 assay read at 450 nm (n = 4). Digitonin was used as a positive cytotoxic control. Values are expressed as mean ± S.E.M. Statistically significant differences relative to PDGF controls (PDGF-stimulated) are indicated by *P < 0.05 and **P < 0.01.