Figure 3

Emodin inhibits adipogenesis differentiation of BMSC. It (0.1-10 μM) was added to the AM culture of BMSC for 7, 14, 21 days. Quantitative PCR (qPCR) assay was performed to examine the expression of adipogenesis-specific genes after treatment with emodin. A) Emodin decreased the expression of PPARγmRNA in BMSC in a dose-dependent manner, with more pronounced effect at the concentration of 10 μM. B) Treatment with emodin at the concentration of 10 μM also significantly decreased the expression of C/EBPαmRNA in osteogenesis differentiation of BMSC for 14 days and 21 days. C) Emodin dose-dependently enhanced the ap2 in adipogenesis differentiation of BMSC, especially at the concentration of 10 μM. D) Western blotting analyses of the protein abundance of PPARγ. β-actin was used as an invariant control for equal loading. P < 0.05 vs OVX group. E) The number of lipid droplets that was formed from the BMSCs of emodin-OVX was significantly lower than that formed from the BMSCs of OVX. Data are presented as mean ± SEM. Data presented are three independent experiments with triplicates within each experiment. P < 0.05, significantly different from the OVX group. Oil red O staining also showed treatment with emodin at the concentrations of 0.1 μM and 10 μM inhibited the adipogenesis differentiation of BMSC. *p < 0.05, when compared with the group without emodin (F, G, H, I).