Zyflamend, a polyherbal mixture, down regulates class I and class II histone deacetylases and increases p21 levels in castrate-resistant prostate cancer cells

Table 3 Effects of Zyflamend or TSA on cell proliferation, protein expression of various histone deacetylases and p21 (relative to controls)

Compounds	Zyflamend (200 ug/ml)	TSA (2 μM)
HDAC1	0.49 ± 0.08*	0.39 ± 0.06*
HDAC2	0.64 ± 0.10*	0.93 ± 0.06
HDAC3	1.14 ± 0.13	0.88 ± 0.17
HDAC4	0.56 ± 0.12*	0.38 ± 0.01*
HDAC7	0.62 ± 0.05*	0.68 ± 0.09*
p21	2.38 ± 0.39*	6.05 ± 0.65*
Relative cell proliferation	0.53 ± 0.11*	0.45 ± 0.04*

Values are mean ± SEM; n = 5/group. ^*Values with an asterisk are significantly different from control at p < 0.05. Abbreviations: HDACs, histone deacetylase; TSA, trichostatin A.

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