Figure 6

Effects of the overexpression of p21 on cell growth in the presence or absence of Zyflamend. CWR22Rv1 cells were transfected with pRc/CMV (empty vector) or pRc/CMV-p21 and treated in the presence or absence of Zyflamend for 24 hr. Relative expression of p21 (protein) is presented in the insert. Following transfection with the empty vector (pRc/CMV) or pRc/CMV-p21 (p21), cells were treated ± Zyflamend for 0–96 hr. Cell proliferation was determined using the MTT assay. Control, open bar, Control + Zyflamend (Zyf), light grey bar; Control + pRc/CMV-p21 (p21), dark grey bar; Zyflamend + pRc/CMV-p21 (p21), black bar. Data are presented as the mean ± SEM. *p < 0.05, ** p < 0.005, †p < 0.0005, ‡p < 0.00005 and §p < 0.000005; (n = 8).