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Figure 4 | BMC Complementary and Alternative Medicine

Figure 4

From: Semi-purified extracts of Commelina benghalensis (Commelinaceae) induce apoptosis and cell cycle arrest in Jurkat-T cells

Figure 4

Inhibition of cell cycle progression in Jurkat-T cells after treatment. The cells were incubated with or without the F1 fraction for 24, 48 and 72 hours. Cells were fixed with 70% ethanol, stained with propidium iodide and then the cell cycle distribution was analyzed. The cells were released from synchronization prior to treatment with the A. F1 fraction. A = serum starved cells for 24 hours, B = control cells, C = cells treated with F1 fraction at 10 μg/mℓ, D = cells treated with F1 fraction at 32.5 μg/mℓ, E = cells treated with F1 fraction at 40 μg/mℓ. B. F2 fraction. A = serum starved cells for 24 hours, B = control cells, C = cells treated with F2 fraction at 30 μg/mℓ, D = cells treated with F2 fraction at 56 μg/mℓ, E = cells treated with F2 fraction at 90 μg/mℓ. Note the distinct accumulation of cells in the S phase. These results are representative profiles of the cell division cycle at 72 hours.

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