Effect of EBT on systemic anaphylaxis and IgE/antigen-induced PCA reaction. (A) Mice were given an intraperitoneal injection of compound 48/80 before EBT was orally administered. (B) EBT was administered orally 1 h prior to the challenge with antigen. Each amount of dye was extracted and measured as described in Materials and methods. The data were expressed as the mean ± SEM (n = 6). ###: P < 0.001 significantly different from the IgE/antigen unsensitized mice mice. *: P < 0.01, **: P < 0.05 significantly different from the IgE/antigen sensitized mice as determined by a one-way ANOVA.