Figure 3
Effects of extracts A. muricata on ROS production on HL-60 cells; cells were treated with extract for 24 h followed by staining with DCHFH 2 -DA (50 μM), incubated for 30 min and the fluorescence in the cells was immediately analyzed using flow cytometry. Data are presented the fluorescence intensity; (A): Leaf; (B): Roots; (C): twigs: (D); Variation of ROS production of extracts of A. muricata; values are expressed as means ± standard error (n = 3). Values affected with different letters are significantly different (p < 0.05) from the control. L: Leaf; R: Roots; T: twigs, Ctl: control; app: apoptic phase.