Figure 2From: Antiproliferative activity and induction of apoptosis by Annona muricata (Annonaceae) extract on human cancer cells Effect of extracts A. muricata on nuclear morphological changes of HL-60 cells. After 24 h of treatment cells staining with Hoechst 33258 incubated for 30 min, and observed under fluorescence microscope. Olympus,Tokyo, Japan; magnification 200×. Marked morphological changes of cell apoptosis such as condensation of chromatin and nuclear fragmentations were found clearly. Apoptotic cells gradually increased in a dose-dependent manner; (A): Leaf; (B): Roots; (C): twigs.Back to article page