Figure 2From: Inhibitory effects of Stemona tuberosa on lung inflammation in a subacute cigarette smoke-induced mouse model Experimental design of subacute cigarette smoke-induced lung inflammation mouse model. Female C57BL/6 mice were exposed (whole body) to the smoke of five cigarettes, four times per day with 30Â min smoke-free intervals, on 6Â days per week for 2Â weeks. Mice were orally treated with DW on days 0 and 1, and DW, roflumilast (5Â mg/kg), or ST (50, 100, and 200Â mg/kg) on days 2 to 5, and 7 to 12. On day 13, mice were sacrificed to acquire BALF and lung tissues for other analyses.Back to article page