Figure 8

Gene expression of proliferating cell nuclear antigen (PCNA), MKI67 and survivin analyzed by RT-PCR. (A) Gene expression of Caco-2 cells treated with degraded FGKC, DKC and CGKC. (B) Gene expression of HepG2 cells treated with degraded FGKC, DKC and CGKC. (C) Gene expression of Caco-2 and HepG2 cells treated with tamoxifen. GAPDH was used as the control gene. Degraded k-carrageenan was found inactivate the proliferating gene in HepG2 cells but not in Caco-2 cells. (A) &(B) Lane 1–4: untreated cells. Lane 5–8: FGKC. Lane 9–12: DKC and lane 13–16: CGKC. Lane 1, 5, 9 & 13: GAPDH. Lane 2,6,10% 14: PCNA. Lane 3,7,11 & 15: MKI67. Lane 4, 8, 12 & 16: Survivin. (C) Lane 1–4: Caco-2 cells. Lane 5–8: HepG2 cell.