Skip to main content
Figure 4 | BMC Complementary and Alternative Medicine

Figure 4

From: Attenuation of inflammatory-mediated neurotoxicity by Saururus chinensis extract in LPS-induced BV-2 microglia cells via regulation of NF-κB signaling and anti-oxidant properties

Figure 4

Effect of the SC extract on nuclear factor (NF)- κB activity in LPS-stimulated BV- 2 microglia. BV-2 microglia cells were seeded at a density of 1 × 105 cells/well on a 12-well plate. BV-2 microglia cells were stimulated with 100 ng/mL LPS in the absence or presence of the SC (10 μg/mL) extract that had been added 1 h before stimulation. At 30 min after LPS stimulation, sub-cellular location of the NF-κB p65 subunit was determined by double-immunofluorescence labeling assay (A). Cells were treated with the indicated dose of SC 30 min before LPS (100 ng/mL) treatment. Total protein was subjected to 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by Western blotting using anti-IκB-α and anti-p (phospho)-IκB-α (B). Densitometric analysis of pIκB-α and IκB-α are represented in the panel. Results are expressed as a ratio of phospho/nonphospo IκB-α/β-actin (C). Data are mean ± standard error (n = 3) of three independent experiments. $p < 0.001, compared with control group; *p < 0.05 and ***p < 0.001 compared with LPS-treated group. SC: Saururus chinensis, LPS: lipopolysaccharide.

Back to article page