Electro-acupuncture treatment changed BAT-related gene expression and Pparγ acetylation in WAT.(A), COX4il (B), or Nrbf1 (C) gene expression in Epi-WAT was measured by qPCR, Data were expressed as means ± SD, *P < 0.05 vs control, **P < 0.01 vs control, n = 12 of each group. (D) Immunobloting for protein expressions of Pparγ, SirT1, and Prdm16 in mice epididymis white adipose tissue, n = 3 in each group. Experiment was repeated 3 times independently. (E) Acetylation of Pparγ in mice white adipose tissue. Experiment was repeated 3 times independently.