Effects of EAW on t-BHP-induced cytotoxicity and apoptosis in PC12 cells. PC12 cells were pretreated with EAW (0, 10, 25 and 50 μg/mL) for 24 h and then treated with or without 100 μM of t-BHP for 3 h. (A) The cytotoxicity was determined by the LDH assay kit. (B) The DNA content was analyzed by the flow cytometry as described in the text (upper panel). The percentage of apoptotic cells (sub-G1 phase) is expressed as mean ± SD (n = 3). ## P < 0.01, compared to the solvent control (0.1% DMSO). *P < 0.05 and **P < 0.01, compared to t-BHP treatment alone.