DPPH quenching effect and HPLC analysis of Wedelia chinesis extract. (A) The indicated concentrations of the ethanol extract, hexane extract (HEW), and ethyl acetate extract (EAW) of Wedelia chinensis were evaluated for DPPH quenching capacity as described in the text. % of DPPH scavengering activity = (absorbance of control group – absorbance of the extract added group)/absorbance of control group × 100%. Means ± SD (n = 3). (B) HPLC analysis of EAW. For the conditions, see the Materials and Methods. Peak 1 is wedelolactone, and peak 2 is luteolin.