Figure 4

Effect of PHY906 on CPT-11-induced inflammation in murine Colon-38 tumor-bearing BDF1 mice with or without (w/o) antibiotic treatment on day 4. (A) Neutrophil infiltration in the middle jejunum section after different treatments. Photographs were taken at 200x magnification. (B and C) qPCR for TNFα and MCP-1 in the middle jejunum after treatment. β-actin was used as an internal control. Each spot represents the mean from two or three different experiments (triplicate samples of each; N = 5). (D-F) Detection of TNF-α, G-CSF and IL6 protein in the plasma after different treatments. (Each spot represents the mean from triplicate samples of each plasma sample; N = 5). Student's t-test was used to determine whether differences between treatment groups were significant. Details of experimental procedures are given in Methods.