Effect of PHY906 on CPT-11-induced body weight loss and damage of the middle jejunum in murine Colon-38 tumor bearing BDF1 mice with or without (w/o) antibiotic treatment on day 4. (A) Effect of PHY906 in protecting against weight loss induced by CPT-11 with or without (w/o) antibiotic treatment. Error bars indicate standard deviations; N = 5. (B) Hematoxylin and eosin staining for visualizing the formalin fixed sections of the middle jejunum four days after treatments commenced. Photographs were taken at 200x magnification. (C) Effect of PHY906 on the expression of Proliferating Cell Nuclear Antigen (PCNA) following CPT-11 treatment with or without antibiotic treatment. (D-F) qPCR for the mRNA expression of the proposed intestinal stem cell markers Lgr5, Olfm4 and Bmi1 in the middle jejunum under different treatment conditions. β-actin was used as an internal control. Each spot represents the mean of two or three different experiments (triplicate samples of each; N = 5). Student's t-test was used to determine whether differences between treatment groups were significant. Details of experimental procedures are given in Methods.