KSY treatment does not increase tumor cell apoptosis. (a) In the Nu-B model, the apoptotic cells (green) were detected by a TUNEL assay. Five independent fields from each tumor tissue section were analyzed for the numbers of TUNEL-positive cells. The percentage of apoptotic cells is shown in the bottom panel and was determined by the following equation: (the number of apoptotic cells/the total cell number of five fields) × 100%. (b) In the Nu-B model, the expression levels of active caspase 3 and cleaved PARP in tumor tissues were measured by Western blot. The quantification data are also shown. *indicates p < 0.05 versus the vehicle group.