Figure 3

Immunohistochemical analysis of VEGF, MMP-2 and MMP-9 expression in liver metastases. (A) H-E staining of liver metastasis ×4, 10, 20 magnification; (B) Detection for the expression of VEGF, MMP-2 and MMP-9 by streptavidin-peroxidase (SP) conjugated method, ×40 magnification; (C) With optical density analysis of liver tumor sections by ImageJ software, the expression of VEGF was reduced by 28.3±2.2% and 63.2±5.7%, MMP-2 by 19.8±1.7% and 59.2±6.3% as well as MMP-9 by 25.7±2.4% and 50.1±5.2% in HCS-L and HCS-H groups respectively, compared to the Control (P<0.05), by Mann–Whitney test. Data is mean±SD.