Figure 4
Representative recordings of the responses to: A, B. jararacussu venom (40 μg/mL) showing its characteristic irreversible neuromuscular blockade (even after washing of preparation); B, pre-incubation of 40 μg/mL venom and 1 mg/mL extract during 30 min before addition into the bath; C, Extract addition (1 mg/mL, at 10 min, black arrow) in venom-pretreated preparation (40 μg/mL). Blue arrows: start of experiment (zero time). Black arrow: extract addition. W = washing. Vertical bar = tension.