Figure 1

Thin Layer Chromatography performed by using ethyl acetate:formic acid:acetic acid:water (100:11:11:24) as solvents. Developer: NP/PEG. Phytochemical standards (inside circles): 1 – mixture of commercial phytochemicals (chlorogenic acid, rutin, quercetin caffeic acid); 2 – V. flavicans extract; 3 – chlorogenic acid (Rf = 0.60); 4 – rutin (Rf = 0.48); 5 – quercetin (Rf = 0.99); 6 - V. flavicans extract, showing Rf values of 0.99, 0.89, 0.80, 0.74, 0.70, 0.66, 0.60, 0.49, 0.23 and 0.13; 7 – caffeic acid (Rf = dragged spot). V. flavicans extract spots are suggestive of several flavonoids (yellow/orange fluorescence) and phenolics constituents (blue fluorescence), including quercetin and chlorogenic acid, respectively. Rf = retention factor.