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Table 3 Phytotoxic effect of the crude extract, subsequent fractions and crude saponins of P. hydropiper against radish seeds

From: Heavy metals analysis, phytochemical, phytotoxic and anthelmintic investigations of crude methanolic extract, subsequent fractions and crude saponins from Polygonum hydropiper L

Samples Conc. mg/ml Average root length (mm) mean ± SEM Average no of seeds inhibited mean ± SEM Root length inhibition % mean ± SEM IC50μg/ml Seeds inhibition % mean ± SEM IC50μg/ml
Ph.Cr 1000 5.13 ± 0.20 17.00 ± 0.58 82.38 ± 0.68*** 16 68.00 ± 2.8*** 310
  500 6.87 ± 0.26 14.66 ± 0.89 76.41 ± 0.89***   58.64 ± 3.5***  
  250 8.23 ± 0.20 11.33 ± 0.89 71.74 ± 0.68***   45.32 ± 2.8***  
Ph.Hex 1000 5.33 ± 0.49 19.33 ± 0.33 82.70 ± 1.6***   77.32 ± 1.3***  
  500 5.03 ± 0.23 17.33 ± 0.67 81.73 ± 0.78*** 124 69.32 ± 2.7*** 215
  250 12.03 ± 0.48 12.33 ± 0.67 58.70 ± 1.60***   49.32 ± 2.7***  
Ph.Chf 1000 3.9 ± 0.52 21.67 ± 0.89 86.61 ± 1.70***   86.68 ± 3.5***  
  500 8.4 ± 0.33 19.00 ± 1.15 71.16 ± 1.10*** 145 76.00 ± 4.6*** 35
  250 11.3 ± 0.43 18.00 ± 0.00 61.20 ± 1.40***   72.00 ± 0.0***  
Ph.EtAc 1000 2.23 ± 0.40 22.33 ± 1.20 92.34 ± 1.30ns   89.32 ± 4.8***  
  500 4.9 ± 0.43 21.66 ± 1.20 83.17 ± 1.40*** 11 85.32 ± 4.8 ns 50
  250 5.9 ± 0.20 17.67 ± 0.89 79.74 ± 0.68*   70.68 ± 3.5***  
Ph.Bt 1000 4.0 ± 0.37 21.33 ± 0.89 86.26 ± 1.25***   85.32 ± 3.5***  
  500 5.93 ± 0.49 18.00 ± 0.58 79.64 ± 1.60*** 8 72.00 ± 0.0*** 100
  250 7.03 ± 0.23 16.00 ± 0.00 75.86 ± 0.78***   64.00 ± 0.0***  
Ph.Aq 1000 6.2 ± 0.23 18.33 ± 0.89 78.71 ± 0.78***   73.32 ± 3.5***  
  500 8.8 ± 0.37 12.67 ± 0.89 69.79 ± 1.20*** 170 50.68 ± 3.5*** 485
  250 12.9 ± 0.48 07.00 ± 1.15 55.71 ± 1.60***   28.00 ± 4.6***  
Ph.Sp 1000 5.00 ± 0.50 22. 00 ± 1.10 91.90 ± 0.75 ns   89.25 ± 2.2***  
  500 7.25 ± 0.20 19.00 ± 1.50 82.10 ± 1.50*** 10 77.25 ± 1.0*** 60
  250 8.50 ± 0.58 17.00 ± 0.89 79.74 ± 0.68*   70.68 ± 1.5***  
Positive control 1000 1.93 ± 0.49 24.67 ± 0.89 93.64 ± 1.60   98.61 ± 1.70  
  500 3.03 ± 0.23 22.16 ± 1.10 89.20 ± 1.40 0.89 88.22 ± 0.23 0.89
  250 5.71 ± 0.78 21.90 ± 0.52 83.33 ± 1.20   87.93 ± 0.49  
Negative control ——— 29.13 ± 0.37 ——— —— —— —— ——
  1. Standard drug (P. Control) Paraquat IC50 = 0.89 μg/ml. Results expressed as average seeds inhibition, % inhibition and Inhibition of root length (mean ± SEM n = 3) and IC50. Values significantly different as compare to Standard drug; *P < 0.05, *** P < 0.001.