Effect of heat treatment of KAOD on hepatoprotection. Serum and livers were isolated from ICR mice fed heat-treated AIN-93G or heat-treated KAOD ad libitum from day 7. (A) The activities of ALT, AST, and LDH in serum were assessed using commercially available kits. Data are presented as mean ± standard deviation, n =6. Significant difference between heat-treated AIN-93G and heat-treated KAOD: *p <0.05; **p <0.01. (B) HE stained liver sections from mice administered (a) heat-treated AIN-93G with CCl4, and (b) heat-treated KAOD with CCl4 were observed under a normal light microscope. Scale bar =100 μm.