Effect of cultivation conditions of the hepatoprotective activities of A. brasiliensis . Serum and livers were isolated from ICR mice fed AIN-93G, KAOD-3%, KAOD-10%, KAID-3%, or JAID-10% ad libitum for 11 days. (A) ALT, AST, and LDH activities in the sera from (a) KAID-3% or KAOD-3% administered mice, or (b) KAOD-10% or JAID-10% administered mice were measured using commercially available kits. Data are presented as mean ± standard deviation, (a) n =6, (b) n =7. Significant difference from AIN-93G or (a) between KAID-3% and KAOD-3% or (b) JAID-10% or KAOD-10%: *p <0.05; **p <0.01; ***p <0.001; n.s.: not significant. (B) HE stained liver sections from mice administered (a-1) AIN-93G with CCl4 (control for KAOD-3% and KAID-3%), (a-2) KAOD-3% with CCl4, (a-3) KAID-3% with CCl4, (b-1) AIN-93G with CCl4 (control for KAOD-10% and JAID-10%), (b-2) KAOD-10% with CCl4, (b-3) JAID-10% with CCl4,. Scale bar =100 μm.