Fuzheng Huayu recipe (FZHY) inhibits Act D/TNF-α induced hepatocyte apoptosis via regulating the apoptotic and anti-apoptotic proteins in mitochondria. Primary hepatocytes were pretreated with 10% FZHY medicated resum or 10% rats’ serum plus 50 μM Z-VAD-FMK for 18 h and then incubated with 200 ng/ml ActD and 20 ng/ml TNF-α for 6 h. (A) Western blot analysis of TNFR1, Bcl-2 and Bax in hepatocytes. TNFR1 expression was low in untreated hepatocytes, whereas TNFR1 expression increased after ActD and TNF-α stimulation. The expression of TNFR1 was significantly down-regulated by FZHY or Z-VAD-FMK treatment. Similarly, the expressions of Bcl-2 and Bax were balanced by FZHY or Z-VAD-FMK treatment. Graphic presentation of the relative expressions of TNFR1 (B) and Bcl-2 and Bax (C). Values were represented as the density of TNFR1, Bcl-2 or Bax vs. housekeeping gene GAPDH (%) from 3 samples. **
P < 0.01 vs. control; ##
P < 0.01 vs. Act D/TNF-α treated group.