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Figure 1 | BMC Complementary and Alternative Medicine

Figure 1

From: Fuzheng Huayu recipe alleviates hepatic fibrosis via inhibiting TNF-α induced hepatocyte apoptosis

Figure 1

Fuzheng Huayu recipe (FZHY) and N-acetylcysteine (NAC) attenuates hepatocyte apoptosis induced by CCl 4 . Mice were respectively treated orally with FZHY (4.0 g/kg) or NAC (0.1 g/kg) daily for 3 d. Then, mice were subcutaneously injected with 100% CCl4 for 18 hours to develop acute liver injury (A). Liver sections were subjected to either hematoxylin-eosin staining to detect necrosis and inflammatory cell infiltration (B, × 100) or to the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay to detect cell apoptosis (C, × 200). (D) Serum ALT and AST levels in the four groups (Control group, n = 10; CCl4 treated, n = 11; FZHY treated, n = 15; NAC treated, n = 15). (E) The apoptotic index (AI) of five high-power fields at × 400 magnification in each tissue specimen. (F) Necrosis and inflammatory cell infiltration scores in the four groups (Control group, n = 10; CCl4 treated, n = 11; FZHY treated, n = 15; NAC treated, n = 15). (G) Quantitative ELISA analysis of hepatic TNF α expression in the four groups (Control group, n = 10; CCl4 treated, n = 11; FZHY treated, n = 15; NAC treated, n = 15). * P < 0.05, ** P < 0.01 vs. control group; # P < 0.05, ## P < 0.01 vs. CCl4 group.

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