The increased HO-1 mRNA and protein expression in tissues after HS were enhanced by VitC. A) HO-1 mRNA in the kidney (a), liver (b), and lung (c) are expressed relative to the level measured in Sham group. B) HO-1 protein expression in the kidney (a), liver (b), and lung (c) were detected using Western-blot. Each gel is representative of six independent experiments. Densitometric analysis was used to calculate the normalized protein ratio (HO-1 to β-actin). Tissue samples were collected at 2, 6, 12 and 24 hours after HS operation. Data represents the mean ± SEM. *p < 0.05 vs. the Sham; #
p < 0.05 vs. the HS at the same time point. Sham: the rats which underwent sham operation and received normal saline (NS). HS: the rats which underwent HS operation and received NS. HSV-post: the rats which underwent HS operation and then received VitC immediately.