Time-course effects of EA intervention on the expression of Ang II was investigated by immunohistochemistry assay in brain tissue after MCAO. Positive expressions of Ang II and its receptors were generally identified in the brain tissue. After MCAO insult, a remarkable augmentation in Ang II level was observed immediately after MCAO, and reached a peak at 12 h following MCAO (p < 0.05). The same tendency was obtained in EA group. Which, however, exhibited a significantly lower level at the 1 to 3 h, and after that, the value was significantly increased and peaked at 24 h. N: Control group; M: Model group; A: EA group.