In vivo anti-metastasis effect of COE. (A) Macroscopic appearance of peritoneal tumors was established by intraperitoneal inoculation of SGC-7901 cells. Arrows indicated peritoneal metastatic tumor in each group. Markedly fewer peritoneal metastases were found in mice in high-dose COE group (40 mg/kg) and positive control group (xeloda). (B) Number of peritoneal metastatic nodules (above 1 mm) in each group. High-dose COE group (40 mg/kg) and positive control group (xeloda) significantly suppressed the development of peritoneal metastases. (C) The average body weight of the mice in the COE groups did not decrease significantly throughout the experiment. (D) Western blot analyses confirmed decreased HSP27 expression in COE-treated tumors. β-actin was served as an internal control of protein level. All values are presented as the means±SD, *P <0.05, **P <0.01, as compared with the control group.