The proportion of immunoreactive (IR) neurons observed at the ipsilateral L5 dorsal root ganglion (DRG). Histograms show the proportion of A. transient receptor potential vanilloid type 1 (TRPV1), B. ankyrin type 1 (TRPA1), C. melastatin type 8 (TRPM8) and D. c–Fos IR neurons. Compared with sham operated animals, spinal nerve ligation surgery (SNL) significantly increased the proportion of neurofilament (NF) 200 positive neurons presenting immunoreactivity for TRPV1, TRPA1, TRPM8 and c–Fos. In the NF200 negative neurons, SNL surgery significantly increased the proportion of TRPV1, TRPM8 and c–Fos IR neurons in the dorsal root ganglion. Perineural pretreatment with 0.05 mg or 0.1 mg diluted bee venom (DBV) significantly decreased the proportion of NF 200 positive and negative neurons expressing TRPV1, TRPA1, TRPM8 and c–Fos compared with the control group. Three rats in each group were analyzed. Each bar represents mean (%) ± standard deviation (SD). *p < 0.05 compared with the control group, † p < 0.05 compared with the BV 0.05 mg group.