Vernonia amygdalina simultaneously suppresses gluconeogenesis and potentiates glucose oxidation via the pentose phosphate pathway in streptozotocin-induced diabetic rats

Table 2 Primer sequence and particulars

Gene	Primer	Sequence	Amplicon	Tm (°C)
GK	Forward	5′-CATATGTGCTCCGCAGGACTA-3′	105 bp	61.7
	Reverse	5′-CTTGTACACGGAGCCATCCA-3′
HK	Forward	5′-ACCCACGAAACAACACCATCA-3′	69 bp	79
	Reverse	5′-GACGTACAACAATGGCTCACTAAAG-3′
PFK-1 (L)	Forward	5′-TTACCGATCACCCTCGTTCCT-3′	80 bp	84
	Reverse	5′-TTCCCCTTAGTGCTGGGATCT-3′
PFK-1 (M)	Forward	5′-GTGGACACTGATATGACCATTGG-3′	67 bp	82
	Reverse	5′-ATGGCGTCCACGATCTCTACA-3′
G6Pase	Forward	5′-GGATCTACCTTGCGGCTCACT-3′	132 bp	62.7
	Reverse	5′-TGTAGATGCCCCGGATGTG-3′
F16BP	Forward	5′-CGTCCTATGCTACCTGTGTCCTT-3′	73 bp	81
	Reverse	5′-CCCCTCTTCTCGGGCTCTATTAT-3′
PEPCK	Forward	5′-GCAACTTCTCTCGGCTCGTT-3′	99 bp	62.7
	Reverse	5′-TGGCAGTTCTACTGGGCTACAC-3′
G6PDH	Forward	5′-GATGGCCTTCTACCCGAAGAC-3′	72 bp	82
	Reverse	5′-GCGGATGTCATCCACTGTGA-3′
P13K	Forward	5′-CCTGAAAGAGCTGGTGCTACATT-3′	71 bp	81
	Reverse	5′-GTGTGACATTGAGGGAGTCATTG-3′

ISSN: 2662-7671