Gene | Primer | Sequence | Amplicon | Tm (°C) |
---|---|---|---|---|
GK | Forward | 5′-CATATGTGCTCCGCAGGACTA-3′ | 105 bp | 61.7 |
Reverse | 5′-CTTGTACACGGAGCCATCCA-3′ | |||
HK | Forward | 5′-ACCCACGAAACAACACCATCA-3′ | 69 bp | 79 |
Reverse | 5′-GACGTACAACAATGGCTCACTAAAG-3′ | |||
PFK-1 (L) | Forward | 5′-TTACCGATCACCCTCGTTCCT-3′ | 80 bp | 84 |
Reverse | 5′-TTCCCCTTAGTGCTGGGATCT-3′ | |||
PFK-1 (M) | Forward | 5′-GTGGACACTGATATGACCATTGG-3′ | 67 bp | 82 |
Reverse | 5′-ATGGCGTCCACGATCTCTACA-3′ | |||
G6Pase | Forward | 5′-GGATCTACCTTGCGGCTCACT-3′ | 132 bp | 62.7 |
Reverse | 5′-TGTAGATGCCCCGGATGTG-3′ | |||
F16BP | Forward | 5′-CGTCCTATGCTACCTGTGTCCTT-3′ | 73 bp | 81 |
Reverse | 5′-CCCCTCTTCTCGGGCTCTATTAT-3′ | |||
PEPCK | Forward | 5′-GCAACTTCTCTCGGCTCGTT-3′ | 99 bp | 62.7 |
Reverse | 5′-TGGCAGTTCTACTGGGCTACAC-3′ | |||
G6PDH | Forward | 5′-GATGGCCTTCTACCCGAAGAC-3′ | 72 bp | 82 |
Reverse | 5′-GCGGATGTCATCCACTGTGA-3′ | |||
P13K | Forward | 5′-CCTGAAAGAGCTGGTGCTACATT-3′ | 71 bp | 81 |
Reverse | 5′-GTGTGACATTGAGGGAGTCATTG-3′ |