Skip to main content

Table 2 Primer sequence and particulars

From: Vernonia amygdalina simultaneously suppresses gluconeogenesis and potentiates glucose oxidation via the pentose phosphate pathway in streptozotocin-induced diabetic rats

Gene Primer Sequence Amplicon Tm (°C)
GK Forward 5′-CATATGTGCTCCGCAGGACTA-3′ 105 bp 61.7
  Reverse 5′-CTTGTACACGGAGCCATCCA-3′   
HK Forward 5′-ACCCACGAAACAACACCATCA-3′ 69 bp 79
  Reverse 5′-GACGTACAACAATGGCTCACTAAAG-3′   
PFK-1 (L) Forward 5′-TTACCGATCACCCTCGTTCCT-3′ 80 bp 84
  Reverse 5′-TTCCCCTTAGTGCTGGGATCT-3′   
PFK-1 (M) Forward 5′-GTGGACACTGATATGACCATTGG-3′ 67 bp 82
  Reverse 5′-ATGGCGTCCACGATCTCTACA-3′   
G6Pase Forward 5′-GGATCTACCTTGCGGCTCACT-3′ 132 bp 62.7
  Reverse 5′-TGTAGATGCCCCGGATGTG-3′   
F16BP Forward 5′-CGTCCTATGCTACCTGTGTCCTT-3′ 73 bp 81
  Reverse 5′-CCCCTCTTCTCGGGCTCTATTAT-3′   
PEPCK Forward 5′-GCAACTTCTCTCGGCTCGTT-3′ 99 bp 62.7
  Reverse 5′-TGGCAGTTCTACTGGGCTACAC-3′   
G6PDH Forward 5′-GATGGCCTTCTACCCGAAGAC-3′ 72 bp 82
  Reverse 5′-GCGGATGTCATCCACTGTGA-3′   
P13K Forward 5′-CCTGAAAGAGCTGGTGCTACATT-3′ 71 bp 81
  Reverse 5′-GTGTGACATTGAGGGAGTCATTG-3′