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Table 1 Outline of thermal cycling conditions

From: Vernonia amygdalina simultaneously suppresses gluconeogenesis and potentiates glucose oxidation via the pentose phosphate pathway in streptozotocin-induced diabetic rats

Step Temperature (°C) Time Number of cycles
Initial denaturation 94 10 min 1
Denaturation 94 20 s 40
Annealing 55 20 s
Extension 72 30 s
Final extension 72 10 min 1
Cool down 4 - -