Vernonia amygdalina simultaneously suppresses gluconeogenesis and potentiates glucose oxidation via the pentose phosphate pathway in streptozotocin-induced diabetic rats

Table 1 Outline of thermal cycling conditions

Step	Temperature (°C)	Time	Number of cycles
Initial denaturation	94	10 min	1
Denaturation	94	20 s	40
Annealing	55	20 s
Extension	72	30 s
Final extension	72	10 min	1
Cool down	4	-	-

ISSN: 2662-7671