Figure 2

Effect of Chijongdan on ethidium homodimer stained cells in A549 cells. A549 cells were treated with various concentrations of 50, 100 and 200 μg/ml of Chijongdan for 24 h. (A) Morphology of A549 cells was observed in inverted microscope. (B) A549 cells were stained with DAPI and ethidium homodimer dyes, mounted with mounting medium containing DAPI and then visualized under a Fluoview FV10i confocal microscope (OLYMPUS, Tokyo, Japan).