Effects of isoquercitrin on cell survival and lipid accumulation in H4IIE cells with FFA. (A) H4IIE cells were exposed to various doses of isoquercitrin, and cell viability was measured by MTT tests. Values are expressed as the percentage of the control, which was taken as 100%, and shown as the mean ± SEM (n = 3). (B) and (C) Cells were cultured in the absence or presence of isoquercitrin (50, 100, and 200 μM) for 24 h, followed by stimulation with or without FFA (0.1 mM) for another 24 h. (B) Intracellular oil droplets were visualized by Oil Red O staining under a microscope at 200 × original magnification. (C) Quantitative analysis of lipid deposition was measured by optical density (OD) values at 540 nm after staining. Values are presented as a dose-dependent decrease and expressed as mean ± SEM (n = 3). **P < 0.01 compared to FFA-treated control group.