Figure 4

Effects of EEOS on downregulation of OPN and CD44 and suppression of PI3K/Akt, COX-2 and VEGF in OPN treated NCI-H460. (A) Effect of EEOS on expression of osteopontin or CD44 in NCI-H460 cells exposed to EEOS for 24 h and Western blotting was performed with antibodies of OPN and CD44. (B) Effect of EEOS with treatment of OPN on expression of CD44 in NCI-H460 cells. Western blotting was performed with antibodies of CD44. (C) Effect of EEOS on expression of PI3K, COX-2 and the phosphorylation of Akt in NCI-H460 cells. (D) Effect of EEOS with treatment of OPN for 30 min or 24 h on expression of PI3K, and the phosphorylation of Akt in OPN treated NCI-H460 cells. (E) Effect of EEOS on VEGF production in NCI- H460 cells. The level of VEGF in NCI-H460 cells was measured by ELISA. *P < 0.05, **P < 0.01, versus control.