Figure 4

Identification of ATF3 promoter sites responsible for GL-induced ATF3 activation. (A). Each indicated construct of the ATF3 promoter (0.5 μg) was co-transfected with 0.05 μg of pRL-null vector into SW480 cells, and cells were treated with 100 μg/ml of GL for 24 h. (B) Each deletion construct (0.5 μg) of the ATF3 promoter was co-transfected with 0.05 μg of pRL-null vector into SW480 cells and cells were treated with 100 μg/ml of GL for 24 h. Luciferase activity was measured. *P <0.05 compared to cells without GL treatment.