Discovery of potential anti-infectives against Staphylococcus aureus using a Caenorhabditis elegans infection model

Table 3 In vitro MIC and MBC values of all positive extracts (A) and compounds (B) against S. aureus

(A) No.	Extract ID	Standard medium (TS broth)		Screening medium (20% TS broth + 80% M9)
		Concentration (μg/mL)		Concentration (μg/mL)
		MIC	MBC	MIC	MBC
1	UE-01-1	<125	1000	<125	500
2	UE-01-2	2000	-	2000	-
3	UE-01-4	-	-	-	-
4	UE-01-6	2000	-	2000	-
5	UE-01-7	1000	2000	500	2000
6	UE-02-4	-	-	-	-
7	UE-03-3	-	-	-	-
8	UE-03-5	-	-	-	-
9	UE-03-6	-	-	-	-
10	UE-08	<125	<125	<125	<125
11	UE-11	-	-	-	-
12	UE-12	-	-	-	-
13	UE-15	1000	2000	500	2000
14	UE-18	1000	-	500	-
(B) No.	Compound ID	Standard medium (TS broth)		Screening medium (20% TS broth + 80% M9)
		Concentration (μM)		Concentration (μM)
		MIC	MBC	MIC	MBC
1	UC-01	125	500	125	500
2	UC-02	2000	2000	2000	2000
3	UC-03	2000	2000	1000	2000
4	UC-06	2000	2000	1000	2000
5	UC-07	200	1000	125	1000
6	UC-08	1000	2000	1000	2000
7	UC-09	2000	2000	1000	2000
8	UC-10	-	-	-	-
9	UC-11	200	-	200	-
10	UC-12	1000	-	1000	-
11	UC-15	<62.5	<62.5	<62.5	<62.5
12	UC-16	2000	-	1000	-
13	UC-27	200	200	200	200
14	UC-28	200	200	200	200

The negative (DMSO) and positive controls (gentamicin) were run in parallel in all replicates. The MIC of gentamicin towards S. aureus was 1.2 μg/mL and MBC was 2.4 μg/mL.
MIC, minimum inhibitory concentration and MBC, minimum bactericidal concentration.

ISSN: 2662-7671