Criteria | Agar-based | Liquid-based |
---|---|---|
Positive hits identified | 1 out of 18 natural extracts screened | 7 out of 18 natural extracts screened |
Average time for media preparation | 2-3Â days | <30Â minutes |
Possibility of compound degradation | Addition of compound into the molten agar at high temperature may cause compound degradation | Does not involve high temperature. Thus, degradation of compound is not possible |
Exposure to compound | Compound is restricted to the agar media, resulting in limited worm exposure to the compound | Worms are bathed in a homogenous solution of compound |
Counting efficiency | Time-consuming to locate the worms on a larger surface area | Smaller surface area and clear distinguishable phenotype of the worms allows quick scoring |
Possible agar color changes complicate worm scoring | Colorless M9 buffer eases the scoring of worms |