|
Tissue
|
Parameter
|
Control
|
LPS
|
Rooibos
|
Rooibos + LPS
|
|---|
|
Blood
|
CAT
|
0.21 ± 0.05
|
0.22 ± 0.02
|
0.27 ± 0.04
|
0.20 ± 0.04
|
|
SOD
|
22.35 ± 3.82
|
22.25 ± 3.25
|
25.46 ± 3.30
|
19.24 ± 1.72
|
|
GR
|
0.15 ± 0.01
|
0.09 ± 0.04*
|
0.15 ± 0.03
|
0.12 ± 0.05##
|
|
GPx
|
0.18 ± 0.03
|
0.14 ± 0.02*
|
0.23 ± 0.03
|
0.16 ± 0.03##
|
|
GSH
|
906.11 ± 173.54
|
596.04 ± 135.59*
|
905.09 ± 135.20
|
862.60 ± 112.25#
|
|
GSSG
|
173.47 ± 34.25
|
205.29 ± 8.69*
|
185.45 ± 35.99
|
202.92 ± 17.52
|
|
GSH:GSSG
|
4.76 ± 1.22
|
2.90 ± 0.65*
|
5.18 ± 1.86
|
4.27 ± 0.59#
|
|
Liver
|
CAT
|
0.11 ± 0.01
|
0.23 ± 0.03*
|
0.11 ± 0.03
|
0.16 ± 0.03#
|
|
SOD
|
55.01 ± 5.27
|
42.06 ± 6.26*
|
61.50 ± 3.85
|
57.62 ± 6.92#
|
|
GR
|
3.99 ± 1.12
|
2.69 ± 0.62
|
3.80 ± 0.80
|
3.37 ± 0.79
|
|
GPx
|
0.16 ± 0.02
|
0.12 ± 0.02*
|
0.16 ± 0.01
|
0.15 ± 0.01#
|
|
GSH
|
6.38 ± 0.58
|
6.37 ± 0.83
|
7.29 ± 0.96
|
7.41 ± 0.75
|
|
GSSG
|
0.32 ± 0.10
|
0.46 ± 0.09*
|
0.28 ± 0.04
|
0.35 ± 0.08#
|
|
GSH:GSSG
|
21.42 ± 6.32
|
13.64 ± 2.39*
|
25.64 ± 4.66
|
21.98 ± 5.34#
|
- Values in columns are mean ± SD (n = 9-10). *Significantly different from control (P < 0.05). #Significantly different from LPS (p < 0.05). ##Marginally different from LPS (p < 0.1). CAT (catalase, μmole H2O2 consumed/min/μg protein), GR (glutathione reductase, μmole NADPH oxidized/min/μg protein), SOD (superoxide dismutase, U/mg protein), GPx (glutathione peroxidase, nmole NADPH oxidized/min/μg protein). GSH (reduced glutathione, μmole/L in blood, μmole/g tissue in liver), GSSG (oxidized glutathione, μmole/L in blood, μmole/g tissue in liver), LPS (lipopolysaccharide).
