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Table 5 The effect of rooibos supplementation on antioxidant enzymes activity and glutathione redox status in the blood and liver of all experimental rats

From: Amelioration of lipopolysaccharide-induced liver injury by aqueous rooibos (Aspalathus linearis) extract via inhibition of pro-inflammatory cytokines and oxidative stress

Tissue Parameter Control LPS Rooibos Rooibos + LPS
Blood CAT 0.21 ± 0.05 0.22 ± 0.02 0.27 ± 0.04 0.20 ± 0.04
SOD 22.35 ± 3.82 22.25 ± 3.25 25.46 ± 3.30 19.24 ± 1.72
GR 0.15 ± 0.01 0.09 ± 0.04* 0.15 ± 0.03 0.12 ± 0.05##
GPx 0.18 ± 0.03 0.14 ± 0.02* 0.23 ± 0.03 0.16 ± 0.03##
GSH 906.11 ± 173.54 596.04 ± 135.59* 905.09 ± 135.20 862.60 ± 112.25#
GSSG 173.47 ± 34.25 205.29 ± 8.69* 185.45 ± 35.99 202.92 ± 17.52
GSH:GSSG 4.76 ± 1.22 2.90 ± 0.65* 5.18 ± 1.86 4.27 ± 0.59#
Liver CAT 0.11 ± 0.01 0.23 ± 0.03* 0.11 ± 0.03 0.16 ± 0.03#
SOD 55.01 ± 5.27 42.06 ± 6.26* 61.50 ± 3.85 57.62 ± 6.92#
GR 3.99 ± 1.12 2.69 ± 0.62 3.80 ± 0.80 3.37 ± 0.79
GPx 0.16 ± 0.02 0.12 ± 0.02* 0.16 ± 0.01 0.15 ± 0.01#
GSH 6.38 ± 0.58 6.37 ± 0.83 7.29 ± 0.96 7.41 ± 0.75
GSSG 0.32 ± 0.10 0.46 ± 0.09* 0.28 ± 0.04 0.35 ± 0.08#
GSH:GSSG 21.42 ± 6.32 13.64 ± 2.39* 25.64 ± 4.66 21.98 ± 5.34#
  1. Values in columns are mean ± SD (n = 9-10). *Significantly different from control (P < 0.05). #Significantly different from LPS (p < 0.05). ##Marginally different from LPS (p < 0.1). CAT (catalase, μmole H2O2 consumed/min/μg protein), GR (glutathione reductase, μmole NADPH oxidized/min/μg protein), SOD (superoxide dismutase, U/mg protein), GPx (glutathione peroxidase, nmole NADPH oxidized/min/μg protein). GSH (reduced glutathione, μmole/L in blood, μmole/g tissue in liver), GSSG (oxidized glutathione, μmole/L in blood, μmole/g tissue in liver), LPS (lipopolysaccharide).