Western blot analysis of PI3K/Akt and MAPK/Erk pathways in MDA-MB-231 cells. (A) Effect of F1 or F2 fractions on the expression of Akt, p-Akt, Erk, p-Erk proteins in MDA-MB-231 cells. Cells were treated with 25 and 50 μg/ml of F1 or F2-fractions and control cells treated with 0.5% DMSO for 48 hours. Western blots were representative of three independent experiments. (B) The densitometer-intensity data of the proteins of each blot is presented as mean ± SEM from three independent experiments. *denotes P < 0.05 versus control (0.5% DMSO) as measured by one-way ANOVA.